We offer Peripherin Antibodies for use in common research applications: ELISA, Immunocytochemistry/Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Sandwich ELISA, Western Blot. Each Peripherin Antibody is fully covered by our Guarantee+, to give you complete peace of mind and the support when

av B Sundström · 1990 · Citerat av 2 — radionuclides and the biological half-life for the antibody was e stimated to over of epithelial cells, the neurofilaments of neuronal cells and the peripherin in.

Validated for IHC,WB. Tested on 20 paraffin-embedded human tissues. Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990) 57:235-248], containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE. Protein Names.

Peripherin antibody

Material Safety Data Sheet or SDS for Anti-Peripherin Antibody AB1530 from MilliporeSigma for download or viewing in the browser. PathPlus™ Peripherin Antibody LS-B12453 is a pathologist validated IHC antibody Rabbit anti-Human Peripherin. Validated for IHC,WB. Tested on 20 paraffin-embedded human tissues. Electrophoretically pure trp-E-peripherin fusion protein [Dev. Brain Res. (1990) 57:235-248], containing all but the 4 N terminal amino acids of rat peripherin. Fusion protein purified from bacterial inclusion bodies by DEAE-cellulose chromatography in 6 M urea followed by preparative SDS-PAGE.

Subsequently, peripherin was found in Anti-Peripherin antibody (ab4666) at 1 µg/ml + Recombinant Human Peripherin protein at 0.01 µg Secondary Goat Anti-Rabbit IgG H&L (HRP) preadsorbed at 1/5000 dilution Developed using the ECL technique. Performed under reducing conditions.

Anti-Peripherin Antibody, clone 8G2 is an antibody against Peripherin for use in IC, IH & WB. Immunocytochemistry: 4% PFA fixed cells (5 minutes) or acetone is recommended. Immunohistochemisty: light PFA fixation is necessary as 8G2 is sensitive to heavy formalin fixation. Antigen recovery is enhanced with 0.1% triton in the block only. 1:25-1:200

Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues. Peripherin antibody LS-C204575 is an unconjugated rabbit polyclonal antibody to human Peripherin. Validated for ICC, IHC and WB. Anti-Peripherin Antibody SDS Safety Data Sheet for Anti-Peripherin Antibody AB1530.

The following antibodies were used: Rheb (Cell Signaling #13879, 1:1000), NSE nestin (Abcam #ab6142, 1:100 (IF)), peripherin (Abcam # ab4666, 1:1000),

Peripherin Antibody (A-3): sc-377093 Anti-Peripherin Antibody (A-3) is a mouse monoclonal IgG 2a κ Peripherin antibody, cited in 2 publications, provided at raised against amino acids 21-90 mapping near the N-terminus of Peripherin of human origin Anti-Peripherin Antibody (A-3) is recommended for Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig's disease (Amyotrophic lateral sclerosis) and some Western blot of rat cerebellar lysate showing specific neuronally derived tumors (Roberson et al., 2003).

Peripherin runs at ~57 kDa and so is a little larger than GFAP and vimentin which run at ~50 kDa (Lane 4 shows blotting with Anti-Vimentin Antibody). Full-length recombinant peripherin was injected into laying hens, and then the IgY fraction was purified from hyperimmunized animals. These antibodies have been validated with human, mouse and rat tissues. Peripherin antibody LS-C204575 is an unconjugated rabbit polyclonal antibody to human Peripherin.
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Cells were stained with chicken antibody to peripherin (1:5,000, green), and co-stained with rabbit antibody to vimentin (R-1699-100, 1:2,000, red). Blue: DAPI nuclear stain. The peripherin antibody reveals cytoplasmic filamentous staining in the small PC12 cells, while the vimentin antibody stains intermediate filaments in the surrounding fibroblastic cells which do not express peripherin.

The PRPH (peripherin) gene is mapped to human chromosome 12q13.12.
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L Leickt, Anders Grubb, S Ohlson, Development of monoclonal antibodies members of two families with different mutations in RDS (the peripherin/RDS. gene).

1983), expressed in motor, sensory and sympathetic system neurons as well as neuroendocrine skin carcinomas and certain melanocytic tumors. Invitrogen Anti-Peripherin Polyclonal, Catalog # PA1-10018. Tested in Western Blot (WB), Immunofluorescence (IF), Immunocytochemistry (ICC) and Immunohistochemistry (IHC) applications. This antibody reacts with Human, Mouse, Rat samples. Supplied as 100 µL unpurified antibody (Conc.

Peripherin Antibody (PA1-10018) in WB. Western blot analysis of tissue and cell lysates using Peripherin polyclonal antibody ( Product # PA1-10018) at a dilution of 1:10,000 in green: 1) protein standard, 2) rat spinal cord, 3) mouse spinal cord, 4) pig spinal cord, 5) cow spinal cord, 6) SH-SY5Y cells and 7) PC12 cells.

Antibodies to peripherin can be used in identifying, classifying, and studying neurons throughout the nervous system. Peripherin is also a good diagnostic marker for ballooned axons seen in Lou Gehrig’s disease (Amyotrophic lateral sclerosis) and some neuronally derived tumors (2).

ZERO BIAS - scores, article reviews, protocol conditions and more Great marker for classifying neurons! Our mouse monoclonal Peripherin antibody is total IgG fraction.